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Mediation analyses revealed that 147 and 186 % of the excess NAFLD risk associated with l-carnitine and DMG, respectively, was mediated by TMAO (the P values for the mediating effects were 0021 and 0036, respectively)

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To investigate the mechanism in detail, we next determined the expression of the TSS pathway enzymes with excess hemin, which significantly reduced the expression of CBS, but not that of CSE (Fig

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